lecture 10 lytic bacteriophages (ii). A new mutation arises in linkage disequilibrium with other genes in the genome. Holliday Junction Model 3. Mechanisms of recombination I: Homologous recombination Human Genetics 541 Thomas E. … of E. coli) Screen for inability to acquire a selectable marker Proteins involved in DNA recombination (the E. coli paradigm) Mutation Phenotype RecA RecBC RecD RecF RecJ RecO RecR RecQ RecN RecG RuvA RuvB RuvC PriA PriB PriC DnaT Recombination deficient Reduced recombination Rec+ independent Reduced plasmid recombination • Reduced recombination in RecBC- • as above • as above • as above Reduced recombination in RecBC- Reduced recombination in RuvA-B-C- Reduced recombination in RecG- as above as above Reduced recombination as above as above as above, Chi (Χ) site: • 8 base pair sequence without • symmetry (5’GCTGGTCC) • Greatly stimulates ability of RecBCD • to catalyze recombination RecBCD exonuclease: opens strands • RecBCD binds to DNA at one end or at • a ds breakage point • Moves along the DNA, creating a loop • and degrading the strand with a free • 3’ end via its exonuclease activity • Exonuclease activity is inhibited • upon passing a Chi site of orientation • Upon cessation of exonuclease activity, • the undegraded 3’ end pairs with • homologous sequences on another • DNA molecule, RecA: needed to form triple helix • RecA binds to free strand to form an • extended helical structure. 경원. Genetic Recombination. Definitions. genetic material is exchanged between two different chromosomes or between different regions within the same chromosome Genetic recombination refers to the rearrangement of DNA sequences by the breakage and rejoining of chromosomes or chromosome segments. Document presentation format: On-screen Show (4:3) Company: Utah Valley State College Other titles: Arial Book Antiqua Default Design Mendelian Genetics Gregor Mendel Gregor Mendel Definitions Fertilization PowerPoint Presentation Mendel’s Interpretations Why were all the seeds in the F1 generation round? The PowerPoint PPT presentation: "????????? is the property of its rightful owner. Bacteria Genetics Introduction Chromosome (bacteria are haploid; in other words, they have a single chromosome) Mutations (change in the base sequence of DNA) Extrachromosomal genetic material Plasmid Transposons Genetic recombination : Transfer of DNA from one bacterium to another Introduction Changes in the genome can occur by two processes Mutations Genetic recombination … The process of creating genetically modified (transgenic) organisms through a laboratory ... - Chapter 12: Genetic Engineering Section 1: Modifying the Living World. dna replication, repair and recombination. - Recombinant DNA Cut the plasmid and the human DNA with the same restriction enzyme. - Genetic Technology 13.2 Recombinant DNA Genetic Engineering Selective breeding is one way to increase allele frequency Genetic engineering, or recombinant DNA is ... - Genetic Engineering What is genetic engineering? Free Genetics PowerPoint Template. 3. Great for KS1 KS2 KS3 KS4 and post 16 A level lessonplans, and more. DNA Recombination Roles Types Homologous recombination in E.coli Transposable elements * Homologues found in eukaryotes (Rad51, etc. Motivation for linkage analysis. Times New Roman Arial WP Greek Helve WP MathA Default Design Microsoft PowerPoint Slide PowerPoint Presentation PART I Ig proteins are specified by genetic “cassettes” DNA rearrangement and alternative RNA spicing Ig proteins are specified by genetic “cassettes” DNA rearrangement and alternative RNA splicing Another view…. genetic analysis –, Genetic Algorithm - . Find PowerPoint Presentations and Slides using the power of XPowerPoint.com, find free presentations research about Genetic Physical Mapping PPT Genetic linkage analysis. fogel and hurst. replication. Search space Cross-over Cross-over recombination Mutation Variants of … Presentation Summary : Genetic Recombination Exchange of genes between two DNA molecules Forms new combination of genes on a chromosome Along with mutations, contributes to genetic. ), mitochondria, and chloroplasts. there is much less recombination in, Genetic Transfer & Recombination In Bacteria - . View and Download PowerPoint Presentations on Genetic Recombination PPT. Genetic Genealogy Year in Review – New tools, techniques, and tricks for genetic genealogists are always being created and developed. Genetic Engineering and DNA Technology can be used to. Conjugation 2. • Usually highly specific and • occurs at only one or a few • integration sites on the • chromosome • Excision utilizes both the • integrase and an excisase, • which act at the hybrid • integration sites that flank the • prophage, GCTTTTTTATACTAA Phage integration and excision (con’t) • Lysogenization by lambda phage: • Site-specific recombination between the attP site on phage and • the attB site on bacterial chromosome • attP and attB are dissimilar except for 15 bp core sequence • GCTTTTTTATACTAA • The lambda Int protein is an integrase that promotes site-specific • recombination between 7 internal bases of the core sequence • Excision is via production on integrase (Int) and excisase (Xis), which promote • recombination of the hybrid attP/B and attB/P molecules in the chromosome, hin H2 flagellin Repressor H1 flagellin Examples of site-specific recombination (con’t) 2) Phase variation of Salmonella flagellin genes • Reversible, high frequency (10-4) inversion of DNA sequence that carries • the promoter for one flagellin structural gene and for a repressor of • a second flagellin gene • Occurs by virtue of a DNA invertase called Hin • Promotes site-specific recombination between two closely linked • sites of DNA P Inverted repeats, © 2020 SlideServe | Powered By DigitalOfficePro, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. Find PowerPoint Presentations and Slides using the power of XPowerPoint.com, find free presentations research about Genetic Recombination PPT Two Holliday Junctions 4. Genetic Recombination 1 / 27. genetic recombination in bacteria Powerpoint Presentation Presentation Title : Genetic Recombination In Bacteria Presentation Summary : Bacteria Genetics Introduction Chromosome (bacteria are haploid; in other words, they have a single chromosome) Mutations (change in the base sequence of DNA) the, Recombination and Linkage - . genetic algorithm tutorial Winner of the Standing Ovation Award for “Best PowerPoint Templates” from Presentations Magazine. 1967. meiotic gene conversion in yeast tetrads and the theory of, Hadronization of a QGP via recombination - Kang seog lee. - Genetic Engineering Georgia Performance Standards: Examine the use of DNA technology in forensics, medicine, and agriculture Essential Questions: - Title: Genetic Engineering Author: Allison R. Enns Last modified by: Palatine High School Created Date: 3/31/2001 11:42:06 PM Document presentation format, - Genetic Engineering Amazing Schemes Within Your Genes, Genetic Recombination and Genetic Engineering. Helpful, trusted answers from doctors: Dr. Woods on genetic recombination ppt: This this question health-related or for homework? View and Download PowerPoint Presentations on Genetic Physical Mapping PPT. ?mRNA? It also describes the consequences of such rearrangements, that is, the inheritance of novel combinations of alleles in the offspring that carry recombinant … 13 RECOMBINANT DNA TECHNOLOGY AND GENETIC ENGINEERING, - ch. Genetics and Programming: Genetics History Evolution in real world GA How do you encode a solution? two classes of genetic recombination. Homologous Recombination at the Molecular Level. Genetic Recombination Revealed by Selective Media Colonies of prototrophs on minimal media met- bio- thr+ leu+ thi+ met+ bio+ thr- leu- thi- A B A + B Cells Must Contact Each Other for Mating: the Davis U tube How does genetic recombination occur? Genetic Recombination in Eukaryotes. introduce foreign DNA or mutations into bacteria . Purposes Promotes genetic diversity within a species - within a chromosome causes inversions, deletions, duplications - horizontal exchange introduces new sequences (information) . nobal niraula university of memphis nov 11, 2010. outline. DNA Technology Biotechnology The use or alteration of cells or biological molecules for specific applications Transgenics Transgenic “changed genes” Recombinant DNA DNA from different species mixed together Natural or man-made Whole organisms or cells Possible because the genetic code is universal All life uses the same genetic code (A,T,G,C) Amplifying DNA Need many copies for various … Announcements 1.Student-initiated genetics study group to meet Thursdays at 4pm in either Brooks 203 or 204 for problem solving. Description : Ppt Presentation on Genetic Engineering which is created by Lisa and available for free download Genetic Engineering powerpoint presentation. ... Genetics chapter 5 part 1 vanessawhitehawk. In the lab: . rDNA technology is used in gene cloning. ??DNA????????????DNA??????,??Holliday??? Genetic Recombination Author: Cynthia Last modified by: Cynthia Created Date: 2/29/2008 9:22:30 PM Document presentation format: On-screen Show Company: The … Loading SlideShow in 5 Seconds.. Genetic Recombination PowerPoint Presentation. Basics of linkage and recombination Ayush Jain. People can be tested to diagnose if they have a certain disease and ... - Title: Genetic Engineering Author: Michael Last modified by: jtowner Created Date: 4/7/2008 11:51:22 PM Document presentation format: On-screen Show. chromosomes. Purposes • Promotes genetic diversity within a species • - within a chromosome causes inversions, deletions, duplications • - horizontal exchange introduces new sequences (information) In the lab: introduce foreign DNA or mutations into bacteria map the distance between mutations Genetic Recombination Definition: The breakage and joining of DNA into new combinations • Plays a major role in repair of damaged DNA and mutagenesis • Critical for several mechanisms of phase and antigenic variation, Types: • Homologous recombination (or general recombination) • basic steps • current models • proteins that play a role • practical applications • Nonhomologous recombination (site-specific) • Basic steps • general categories of proteins used • examples – phage integration, flagellin phase variation • Illegitimate recombination (transposition), CACATGATACGTCCGATCACATTTGTTGTTCATAT GTGTACTATGCAGGCTAGTGTAAACAACAAGTATA GTGTACTATGCAGGCTAGTGTAAACAACAAGTATA CACATGATACGTCCGATCACATTTGTTGTTCATAT • Results in the creation of a synapse [synapse is point where DNA strands are held • together by complementary base-pairing (H bonds)] CACATGATACGTCCGATCACATTTGTTGTTCATAT GTGTACTATGCAGGCTAGTGTAAA Synapse C GTATA A A A A C GTATA GTGTACTATGCAGGCTAGTGTAAA CACATGATACGTCCGATCACATTTGTTGTTCATAT Homologous Recombination • Step One • Formation of complementary base pairing between two ds DNA molecules - Sequences must be the same or very similar - 23 base pair minimum, CACATGATACGTCCGATCACATTTGTTGTTCATAT GTGTACTATGCAGGCTAGTGTAAA C GTATA CACATGATACGTCCGATCACATTTGTTGTTCATAT GTGTA A A A A GGCTAGTGTAAACAACAAGTATA C G A C T GTATA GTGTACTATGCAGGCTAGTGTAAA A C CACATGATACGTCCGATCACATTTGTTGTTCATAT T G G T Branch migration A C T A C GGCTAGTGTAAACAACAAGTATA GTGTA CACATGATACGTCCGATCACATTTGTTGTTCATAT Step two Branch migration to extend the region of base-pairing (the heteroduplex) • ATP-hydrolyzing proteins (Ruv proteins) break and re-form H bonds • allow migration to go faster, CACATGATACGTCCGATCACATTTGTTGTTCATAT GTGTACTATGCAGGCTAGTGTAAA C GTATA CACATGAT ACGT CCGATCACATTTGTTGTTCATAT GTGTA A A A A GGCTGGTGTAAACAACAAGTATA C G A C T GTATA GTGTACTATGCAGGCTGGTGTAAA A C CACATGATACGTCCGACCACATTTGTTGTTCATAT T G G T Branch migration A C T A C GGCTAGTGTAAACAACAAGTATA GTGTA CACATGAT AC GT CCGACCACATTTGTTGTTCATAT Branch extension can increase the chance of gene conversion via increasing the chances of including mismatches in the heteroduplex region, Step three Resolution of the heteroduplex - isomerization of the duplex due to strands uncrossing and re-crossing - results in different outcomes upon resolution - 50% chance of each isomer being resolved, Models of Homologous Recombination (I) Holliday double-strand invasion Fig. Multigene organisation of Ig genes 2. Generic Recombination of Biotech Genetic Engineering 遗传工程 gene cloning 基因克隆 recombination DNA technique 重组 DNA 技术 - 第九章 基, Fine recombination mapping in S. cerevisiae using tiling microarrays - . Source : http://ocean.otr.usm.edu/~w536943/Courses/BSC380/Chapter%208c.ppt - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. genetic analysis of phages. FOR EVERY ONE 2. ?po, carrier screening, which involves identifying, newborn screening-phenylketonuria, congenital, Genealogical DNA test (for genetic genealogy, confirmational diagnosis of a symptomatic. Times New Roman Arial WP Greek Helve WP MathA Default Design Microsoft PowerPoint Slide PowerPoint Presentation PART I Ig proteins are specified by genetic “cassettes” DNA rearrangement and alternative RNA spicing Ig proteins are specified by genetic “cassettes” DNA rearrangement and alternative RNA splicing Another view…. 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